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Trapping site 1: Portugal

Universidade Nova de Lisboa

Leading Partner

Sand fly sample collection

Sand fly data collection involved longitudinal entomological surveys conducted from April to November 2023.  

This included the use of CDC light traps for two nights per month across 30 sampling locations. Of these, 14 were situated in the Metropolitan Region of Lisbon (Lx), while the remaining 16 were in the Algarve region (AL).  

Sampling sites included various animal habitats such as hen houses, stables, and kennels. Additionally, data loggers were deployed at each location to monitor temperature and humidity levels every two hours.

Traps used to catch sand flies

The insects are captured at night, and they will be collected to later be analysed in the laboratory.  

Sand flies flight pattern as it can be seen in the video, consists of small hops, which is very characteristic of compared to mosquitoes.

Image credits: © Carla Maia / UNL

Sand fly collection and analysis

The sand flies after being separated from the other insects They are preserved at -80ºC until they are identified.cuando estan en el laboratorio se separa con ice block por debajo de la placa de Petrick para separar los mosquitos, non-targuet speaces y los flebotomos

Image credits: © Carla Maia / UNL

Sand fly specimens
Sand fly specimens

Specimens were captured in a single trap, with a ‘red’ female in the middle.

Red female sand fly

This female will be used, following morphological identification, for the extraction of nucleic acids and detection of Leishmania, and Phlebovirus, and also for identifying the food source.

Sand flies separated

Sand flies, after being separated from other insects, will be preserved at -80°C until they are identified

Sand flies separated
Females and male sand fly

At 12 o’clock and 3 o’clock are females sand fly,  at 6 o’clock is a blood-fed female, and at 9 o’clock is a male sand fly.

Sand flies separated

Preliminary results

Coming soon