Leading Partner
Image credits: © Universidad de Murcia
Transportation & Storage for dead sand flies:
Identification, Leishmania and phlebovirus diagnosis (and possibly, isolation) Sand flies are separated from other insects in a collection pot at -200C/2hrs and stored in groups (same trap and day) in 70% ethanol at -200C until dissection.
Alive Sand flies:
Identification, Leishmania and phlebovirus diagnosis, and phlebovirus isolation
Collection: Place collection pots in cages to gather sand flies using a mouth aspirator.
Temporary Stun: Transfer live sand fly specimens to a beaker and stun them at -20°C for 10 minutes.
Storage: Store sand flies in dry vials at -80°C.
Image credits: © Universidad de Murcia
Thorax and abdomen (stored individually in 70% ethanol at -200C
In his head you can notice a dark structure between his eyes, this structure is called cibarium and it is a taxonomical structure that helps you separate the females from genus sergentomyia and phlebotomus.
In his head you can notice a dark structure between his eyes, this structure is called cibarium and it is a taxonomical structure that helps you separate the females from genus sergentomyia and phlebotomus.
Phlebotomus p. papatasi on top and Phlebotomus p. perniciosus on the bottom. They are different in color (yellowish vs. brownish) and size.
| Region | No. sand flies. |
|---|---|
| Galicia | 27 |
| Basque Country | 15 |
| Aragón | 1080 |
| Castilla La Mancha | 1186 |
| Madrid | 2335 |
| Catalonia | 1505 |
| Murcia | 2699 |
| Andalucía | 356 |
| All | 9203 |
Murcia, Murcia Region
P. papatasi male with supernumerary spine: 3 terminal spines in the lateral lobe
| Species | No. dead SF | No. alive SF |
|---|---|---|
| Phlebotomus perniciosus | 1351 | 59 |
| P. papatasi | 309 | 43 |
| P. sergenti | 2 | 1 |
| P. ariasi | 1 | 0 |
| Sergentomyia minuta | 72 | 5 |
Ciudad Real, Castilla LaMancha
| Species | No. dead sand flies |
|---|---|
| Phlebotomus perniciosus | 602 |
| P. papatasi | 84 |
| P. sergenti | 8 |
| P. perfiliewi? (barcoding) | 2 |
| Other for barcoding | 15 |
| Sergentomyia minuta | 35 |
Total sand flies collected: 15
Alava, Basque Country: Lezama (Cattle shed), Vitoria (Dog kennel), Arkaute (Sheep shed)
Vizcaya, Basque Country: Gernika (Dog kennel)
| Species | No. dead sand flies |
|---|---|
| Phlebotomus ariasi | 14 |
| Phlebotomus mascitii | 1 |
